How do you use DNE?
DNE Technology
DNE takes advantage of a cell's natural ability to repair its genome following the introduction of a DNA break. The cell is first transformed with a gene encoding an engineered DNE meganuclease. The expressed meganuclease cleaves the genomic DNA at the desired locus, which greatly stimulates homologous recombination as part of the cellular DNA-repair response. If the cell is co-transformed with a transgenic DNA sequence with homology to the meganuclease cut site (i.e. a "repair matrix"), the genomic DNA can undergo recombination with the matrix DNA. The result is the incorporation of the matrix sequence into the genome. Depending on the sequence of the matrix DNA, this process can generate a wide range of genetic modifications, including:
- Gene Knockout. Stop and frameshift mutations can be introduced into a gene coding sequence to permanently inactivate the gene.
- Gene Insertion. Entire genes and multi-gene cassettes can be targeted to defined chromosomal locations.
- Gene Repair. Precise genetic modifications can be made to repair defective genes or produce model cells and organisms.
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