Application

BioTools

BioTherapeutics
PlantSciences

"BioTools" refers to applications of DNE as molecular biology or cell engineering reagents.

• BioTools for Molecular Biology. Engineered DNE meganucleases can be used for cloning and molecular analysis purposes in much of the same ways as conventional restriction enzymes. The important difference, of course, is that meganucleases recognize much rarer DNA sequences than restriction enzymes. This makes them particularly well suited to the manipulation of extremely large DNA sequences such as intact genomes. Importantly, DNE meganucleases cleave to leave 4 basepair 3' overhangs suitable for "sticky-end" cloning.
• BioTools for Cell and Animal Engineering. Engineered DNE meganucleases are commonly used to engineer cell lines to generate disease models or to produce optimized cell lines for the biomanufacturing of protein vaccines and therapeutics. The high efficiency of DNE makes it possible to quickly generate cell lines with multiple genetic changes or to produce large numbers of isogenic bioproducer cells without the need to clone from a single cell. DNE also enables the production of genetically engineered animal models that precisely mimic human disease genotypes.